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GM6001 br Conclusion Pu erh extracts showed a
2018-11-12
Conclusion Pu-erh extracts showed a strong protection effect against the hepatic damage by oxidative stress. The protection mechanism of Pu-erh extracts is confirmed in the study via the activation of the hepatic antioxidant system and the reversal of lipid peroxidation. In addition, Pu-erh tea e
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Cell detachment may also result from
2018-11-12
Cell detachment may also result from a weakening of the laminin from the glass cover slip. Previously, Fourier transform infrared characterization on cover slips that were patterned using this method verified the attachment of laminin to the underlying aldehyde. However, in-depth analysis of the spe
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Microarray data was validated using quantitative PCR qPCR
2018-11-12
Microarray data was validated using quantitative PCR (qPCR). This was first performed on a set of genes chosen for their wide variation in gene src kinase inhibitor between the three cell types (Prl2c2, GHR, Zyx, Des) (Supplementary Figure S2). qPCR was then performed to validate the expression of g
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TRPM channel has been implicated in cell survival and
2018-11-12
TRPM7 channel has been implicated in cell survival and proliferation. In zebra fish, TRPM7 knockouts showed several development defects in skeletal muscle and growth retardation (Elizondo et al., 2005). TRPM7 was also reported to regulate cell migration (Wei et al., 2009). Knocking down TRPM7 in DT-
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Both Brat and Mei P belong to the Trim
2018-11-12
Both Brat and Mei-P26 belong to the Trim-NHL family of proteins, which have conserved functions in stem cell lineages from C. elegans to mouse (for review, see Wulczyn et al., 2010). Proteins within this family are potential E3 ubiquitin ligases and can act by either activating or antagonizing the m
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br Results br Discussion In the
2018-11-12
Results Discussion In the field of stem cell research, the demand for standardized media is rising, allowing for better reproducibility of results and thereby deepening our knowledge of the pathways regulating their maintenance and differentiation. Here, we set out to determine the minimum req
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Thus the persistent muscle expression
2018-11-12
Thus, the persistent muscle expression of full length dystrophin remains difficult to achieve currently. Since the discovery of satellite stem px12 and the difficulties met in maintaining their differentiation potential during in vitro culture, several promising alternative candidates for DMD cell t
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Pluripotency tests included flow cytometry analysis of prote
2018-11-12
Pluripotency tests included flow cytometry analysis of protein stem cell markers SSEA3, SSEA4, Tra1-60, Tra1-81 and Oct3/4 (Fig. 2A), alkaline phosphatase staining (ALP) (Fig. 2B), and qRT-PCR analysis of endogenous pluripotent transcription factors OCT-4, SOX-2, NANOG and REX1 (Fig. 2C). To finally
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br Materials and methods br
2018-11-12
Materials and methods Results Discussion The main goal of current investigation was to study the role of oxygen in the maintenance of LESC phenotype under conditions of biochemically undefined and a serum-free highly defined growth environment. The oxygen concentrations used in the present
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order W 54011 To investigate whether PD derived HSCs exist i
2018-11-12
To investigate whether PD-derived HSCs exist in other mammals, we analyzed the resident cells in the peritoneal cavity of C57BL/6 mice without any prior treatment. We found that there was a higher ratio (0.51±0.04%) of Lin−/Sca1+/c-kit+ population in the mouse peritoneal cavity than in bone marrow (
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br Materials and methods The PQ embryos
2018-11-12
Materials and methods The PQ embryos from in vitro fertilization were donated, and the fetal membranes were collected in accord with the INPER Ethics Committee (212250-21071) as well as the Guidelines for the Conduct of hESC Research (ISSCR, 2006). The hAEC were isolated and cultured from human
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br Introduction The mammary tissue undergoes extensive morph
2018-11-12
Introduction The mammary tissue undergoes extensive morphological and phenotypic alterations during the pregnancy/lactation cycle forming a complex network of alveolar structures (Rosen et al., 1994; Macias and Hinck, 2012). This cellular architecture exhibits well defined A/B polarization establ
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br Materials and methods br Author disclosure statement br
2018-11-12
Materials and methods Author disclosure statement Acknowledgments This work was supported by the UK Medical Research Council grants G0701172 and G0801061. We thank Dr. Yacoub Khalaf, Director of the Assisted Conception Unit of Guy\'s and St Thomas\' NHS Foundation Trust and his staff for su
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Dedifferentiation has been considered as
2018-11-09
Dedifferentiation has been considered as one of the mechanisms rerouting cell fate by reverting differentiated cells to an earlier, more primitive phenotype. Interestingly, previous studies from both our group and others have demonstrated that dedifferentiation is a prerequisite for bone marrow stro
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that does not imply adjusting the step length The convergenc
2018-11-09
that does not imply adjusting the step length. The convergence process is often sawtooth-shaped and does not always allow finding the solution in an acceptable number of iterations. To remove this difficulty, we analyzed the methods of adjusting the step length in the relation For the case of a
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